elk 1 fusion protein Search Results


elk 1 fusion protein  (Cell Signaling Technology Inc)
86
Cell Signaling Technology Inc elk 1 fusion protein
Elk 1 Fusion Protein, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/elk 1 fusion protein/product/Cell Signaling Technology Inc
Average 86 stars, based on 1 article reviews
elk 1 fusion protein - by Bioz Stars, 2026-05
86/100 stars
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elk1 fusion protein  (Cell Signaling Technology Inc)
86
Cell Signaling Technology Inc elk1 fusion protein
FIGURE 5. Activity of GFP-ERK2 and its mutants. A, COS7 cells were trans- fected with each of the various constructs, serum-starved, and stimulated with EGF (50 ng/ml, 10 min). Cell lysates were prepared and subjected to immunoprecipitation with monoclonal anti-GFP Ab. The catalytic activity of the different GFP-ERKs was tested by an in vitro kinase assay using <t>Elk-1</t> as the substrate. Each mutant was tested for its catalytic activity in three different concentrations. The concentrations were in a ratio of 1:4:12. Samples were separated by SDS-PAGE and subjected to Western blotting with the indicated Abs. B, COS7 cells were transfected with each of the various constructs, serum-starved, and harvested. The cell lysates were subjected to immunoprecipitation with monoclonal anti-GFP Ab. The GFP-ERK2 immunoprecipitates were subjected to in vitro kinase assay using partially purified MEKs as the kinases. The catalytic activity of the different activated GFP-ERKs was tested by an in vitro kinase assay using MBP as a substrate. The phosphorylation of MBP was detected by autora- diography on an x-ray film. The amounts of the GFP-ERKs were deter- mined by a Western blot analysis with the indicated Abs.
Elk1 Fusion Protein, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/elk1 fusion protein/product/Cell Signaling Technology Inc
Average 86 stars, based on 1 article reviews
elk1 fusion protein - by Bioz Stars, 2026-05
86/100 stars
  Buy from Supplier
elk1 fusion protein  (Proteintech)
N/A
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elk1 protein-gst fusion  (Rockland Immunochemicals)
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ELK1 recombinant protein-GST fusion protein - 009-001-R88
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FIGURE 5. Activity of GFP-ERK2 and its mutants. A, COS7 cells were trans- fected with each of the various constructs, serum-starved, and stimulated with EGF (50 ng/ml, 10 min). Cell lysates were prepared and subjected to immunoprecipitation with monoclonal anti-GFP Ab. The catalytic activity of the different GFP-ERKs was tested by an in vitro kinase assay using Elk-1 as the substrate. Each mutant was tested for its catalytic activity in three different concentrations. The concentrations were in a ratio of 1:4:12. Samples were separated by SDS-PAGE and subjected to Western blotting with the indicated Abs. B, COS7 cells were transfected with each of the various constructs, serum-starved, and harvested. The cell lysates were subjected to immunoprecipitation with monoclonal anti-GFP Ab. The GFP-ERK2 immunoprecipitates were subjected to in vitro kinase assay using partially purified MEKs as the kinases. The catalytic activity of the different activated GFP-ERKs was tested by an in vitro kinase assay using MBP as a substrate. The phosphorylation of MBP was detected by autora- diography on an x-ray film. The amounts of the GFP-ERKs were deter- mined by a Western blot analysis with the indicated Abs.

Journal: Journal of Biological Chemistry

Article Title: Role of Non-phosphorylated Activation Loop Residues in Determining ERK2 Dephosphorylation, Activity, and Subcellular Localization

doi: 10.1074/jbc.m703120200

Figure Lengend Snippet: FIGURE 5. Activity of GFP-ERK2 and its mutants. A, COS7 cells were trans- fected with each of the various constructs, serum-starved, and stimulated with EGF (50 ng/ml, 10 min). Cell lysates were prepared and subjected to immunoprecipitation with monoclonal anti-GFP Ab. The catalytic activity of the different GFP-ERKs was tested by an in vitro kinase assay using Elk-1 as the substrate. Each mutant was tested for its catalytic activity in three different concentrations. The concentrations were in a ratio of 1:4:12. Samples were separated by SDS-PAGE and subjected to Western blotting with the indicated Abs. B, COS7 cells were transfected with each of the various constructs, serum-starved, and harvested. The cell lysates were subjected to immunoprecipitation with monoclonal anti-GFP Ab. The GFP-ERK2 immunoprecipitates were subjected to in vitro kinase assay using partially purified MEKs as the kinases. The catalytic activity of the different activated GFP-ERKs was tested by an in vitro kinase assay using MBP as a substrate. The phosphorylation of MBP was detected by autora- diography on an x-ray film. The amounts of the GFP-ERKs were deter- mined by a Western blot analysis with the indicated Abs.

Article Snippet: Elk1 fusion protein was from Cell Signaling (Beverly, MA).

Techniques: Activity Assay, Construct, Immunoprecipitation, In Vitro, Kinase Assay, Mutagenesis, SDS Page, Western Blot, Transfection, Purification, Phospho-proteomics